Synthesis and characterization of a new MR-visible neuronal tracer.
Ronda L., Piano R., Paredi G., Borra E., Gerbella M., Galati S., Buschini A., Bettati S.
Detailed anatomical information on connectivity of cortical areas is crucial for understanding the neural mechanisms underlying cortical functions. Unfortunately, available techniques providing information on cortical networks in animal studies still do not have reliable non-invasive equivalents for human studies. We prepared and characterized a new neuronal tracer suitable for magnetic resonance imaging where the axonal migration properties of wheat germ agglutinin, a neuronal tracer widely used for animal brain studies, has been coupled with: 1)gadolinium-DOTA, to make it visible in MR imaging and 2) a dansyl solvatochromic fluorescent probe, to characterize its cellular localization and degradation.