Antisecretory Factor modulates GABA-A receptor activity on neuronal cells.

Gatta E., Bazzurro V., Cupello A., Lange S., Robello M.
  Giovedì 14/09   09:00 - 11:30   Aula A209   V - Biofisica e fisica medica
The Antisecretory Factor (AF, Mw 41kD) is an endogenous protein, found in all mammalian tissues. AF acts $in vivo$ by counteracting intestinal hypersecretion and various forms of inflammations. The detailed $in vitro$ AF action on the cellular level is unknown. Using an $in vitro$ neuronal model (granular cells from rat small brain) we studied the AF action on ionotropic GABA-A receptors. A facilitatory activity, due to increased GABA-A receptors expression, was demonstrated on the neuronal plasma membrane in response to AF influence. This effect may result in the inhibition of intestinal secretomotor cells.