Antisecretory Factor modulates GABA-A receptor activity on neuronal cells.
Gatta E., Bazzurro V., Cupello A., Lange S., Robello M.
The Antisecretory Factor (AF, Mw 41kD) is an endogenous protein, found in all mammalian tissues. AF acts $in vivo$ by counteracting intestinal hypersecretion and various forms of inflammations. The detailed $in vitro$ AF action on the cellular level is unknown. Using an $in vitro$ neuronal model (granular cells from rat small brain) we studied the AF action on ionotropic GABA-A receptors. A facilitatory activity, due to increased GABA-A receptors expression, was demonstrated on the neuronal plasma membrane in response to AF influence. This effect may result in the inhibition of intestinal secretomotor cells.