Relazione su invito
3D quantitative investigation of the mouse spinal cord neuronal arrangements for preclinical application using X-ray Phase-Contrast multiscale-Tomography.
Fratini M., Campi C., Bukreeva I., Brun F., Modregger P., Mastrogiacomo M., Requardt H., Giove F., Bravin A., Cedola A.
The investigation of the neuronal network (NN) in mouse spinal cord (SC) models represents the basis for the research on neuro-pathologies. We investigated SC mouse neuronal architecture using X-ray Phase-Contrast Tomography with a spatial resolution of 640 nm, without any aggressive sample preparation or sectioning. The high quality of the obtained images enables a quantitative study of the neuronal structure. We developed and applied a tool based on a spatial statistical analysis of the motor-neurons to obtain quantitative information on their 3D arrangement in the healthy-mice spinal cord and in pathological systems for a comparative investigation of neurodegenerative diseases and therapies.