Simulating Atomic Force Microscopy images from cryo-EM maps of polyribosomes for studying geometrical parameters of the protein synthesis machinery with high-processivity.

Lorenzo L., Viero G.
  Mercoledì 13/09   09:00 - 13:00   Aula A222   V - Biofisica e fisica medica
The most energy consuming process in cells is protein synthesis that takes place on polyribosomes among the biggest machines formed by groups of ribosomes on a mRNA. While the structure of the ribosome has been widely studied, the organization of polyribosomes is not yet studied in detail. Here we used a modelling approach to fit the three-dimensional structure of human ribosomes into cryo-EM maps of disomes in four basic orientations and simulate AFM images. We studied the distribution of distances between next-neighbor ribosomes and compared them to experimental data. This framework allows a principled studying of super-structural organizations of thousands of polyribosomes in a detail and processivity never achieved before.