Role of membrane lipid composition for the activity of two CDCs pore forming toxins.
Marchioretto M., Zanetti M., Lunelli L., Musio C., Anderluh G., Dalla Serra M.
Cholesterol-Dependent Cytolysins (CDC) are important virulence factors of many pathogenic bacteria; they act by punching pores into target cell membranes. By using single-channel recordings, AFM, and DLS techniques, we identified two different kinds of lesions produced by Listeriolysin O (LLO) and Perfringolysin O (PFO). Depending on membrane fluidity both CDCs are able to build partial or full ring-shaped lesions into target membranes. The formation of arc-shaped pores, necessarily induces changes into the lipid lamellar structure, which should rearrange to constitute the arc cord. This is also supported by a detergent-like activity of both toxins in more fluid membranes.